Effects of HMGN1 on Chromatin Structure and SWI/SNF-mediated Chromatin Remodeling

Mechanism of chromatin remodeling.

Results from biochemical and structural studies of the RSC chromatin-remodeling complex prompt a proposal for the remodeling mechanism: RSC binding to the nucleosome releases the DNA from the histone surface and initiates DNA translocation (through one or a small number of DNA base pairs); ATP binding completes translocation, and ATP hydrolysis resets the system. Binding energy thus plays a cen...

The “Dark Side” of Chromatin Remodeling Repressive Effects on Transcription

octamer relative to the DNA. The movement of a histone octamer along the DNA must involve the breaking and reestablishment of histone–DNA contacts within the nucleosome, but exactly how the histone–DNA contacts are altered is not known. In theory, the ATP-driven trans-location of a chromatin remodeling complex along the The packaging of the eukaryotic genome into chromatin DNA could directly br...

Chromatin remodeling factors Isw2 and Ino80 regulate checkpoint activity and chromatin structure in S phase.

When cells undergo replication stress, proper checkpoint activation and deactivation are critical for genomic stability and cell survival and therefore must be highly regulated. Although mechanisms of checkpoint activation are well studied, mechanisms of checkpoint deactivation are far less understood. Previously, we reported that chromatin remodeling factors Isw2 and Ino80 attenuate the S-phas...

SnapShot: Chromatin Remodeling: ISWI

SnapShot: Chromatin Remodeling Complexes

Unique subunits Swp82, Taf14, Snf6, Snf11 Rsc3,5,7,9, 10,30, Htl1, Ldb7, and Rtt102 INO80 regulates transcription and is involved in DNA repair and cellcycle checkpoint adaptation. It has ATP-dependent nucleosome mobilization activity in vitro. SWR1 regulates transcription and is also involved in deposition of histone H2AZ, chromosome stability, and cell-cycle checkpoint adaptation. In vitro it...